[21 Purification and Assessment of Proteins Associated with Nitric Oxide Synthase

By Charles J. Lowenstein

Introduction

Calmodulin was the first polypeptide shown to interact with nitric oxide synthases (NOS).1 A variety of polypeptides have subsequently been identified that interact with NOS (Table I1"18). Some of them, such as calmodulin, interact with all three of the NOS isoforms: neuronal NOS (nNOS or NOS 1), inducible NOS (iNOS or NOS2), and endothelial NOS (eNOS or NOS3). Other proteins interact with

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