[21 Purification and Assessment of Proteins Associated with Nitric Oxide Synthase

By Charles J. Lowenstein

Introduction

Calmodulin was the first polypeptide shown to interact with nitric oxide synthases (NOS).1 A variety of polypeptides have subsequently been identified that interact with NOS (Table I1"18). Some of them, such as calmodulin, interact with all three of the NOS isoforms: neuronal NOS (nNOS or NOS 1), inducible NOS (iNOS or NOS2), and endothelial NOS (eNOS or NOS3). Other proteins interact with

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[02' ] = [aconitaseinactive]^reactivation/[aconitaseactive]Â:inactivation, although many investigators simply report the relative ability of two samples to inactivate aconitase to obtain a comparative measure of O2'- levels.

Acknowledgments

This work was supported by NIH Grants HL38206 (K.K.G.), HL58000 (K.K.G.), HL58863 (K.K.G.), HL62984 (F.M.), and HL03669 (F.M.). We are grateful to Dr. Dan Sorescu and Maijorie Akers for critical reading of the manuscript, and to Carolyn Morris for excellent secretarial assistance.

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