B

Load cells with BioGEE

Oxidant Stress

Extract Soluble Protein

Remove Non-spec if ically Binding Proteins with Biotin-blocked Streptavidin

PROTEiNOS—SGOBIOTINft-%,

Bind Biotinylated Molecule?/ with Streptavidin Agarose

Wash Extensively with Detergent Buffer |

Elute with DTT

PROTEiNOS—SGOBIOTINft-%,

Elute with DTT

PROTEINCS^-SGOBIQTIfsM SA®AGAROSE ^DTT

PROTEINCS^-SGOBIQTIfsM SA®AGAROSE ^DTT

PROTEINeSH

HSGOBIOTIN« 6A0AGAROSE.-

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