Ret

Basic Local Alignment Search Tool, identification of human genes, 569, 572-574 B cell

BALB/c mouse plasmacytoma model genetic susceptibility, 435 induction, 434-435 pristane granuloma role, 435-436 transgenic shuttle vector assays of oxidative mutagenesis in plasmacytomagenesis comparison of assays, 445-447 A.LIZ assay magnetic cell sorting, 440-441 oxidative mutagenesis in lymphoid tissues and purified B cells, 439-441 principles, 437, 439 pristane treatment, 439-440 overview, 436-437 pUR288 assay phagocyte-mediated oxidative mutagenesis, 444-445 principles, 442-444 BH4, see Tetrahydrobiopterin Biotinylated glutathione ethyl ester affinity purification of labeled proteins,

111, 113 metabolism in cells, 113 protein incorporation assay cell loading, 109

lysate preparation, 110 materials, 107, 109 Western blot, 110 rationale for protein labeling, 103-104 structure, 104 synthesis free sulfhydryl determination, 106 materials, 106 overview, 104-105 purification, 106-107 reaction mixture, 106 BLAST, see Basic Local Alignment Search Tool

0 0

Post a comment