C

Caenorhabditis elegans oxidant stress-responsive genes green fluorescent protein reporters and applications, 497-499, 505 oxidative challenge, 497-498 paraquat-inducible glutathione S-transferase promoter green fluorescent protein detection,

502, 505 hyperbaric oxygen as inducer, 502 plasmid design, 499, 501 transgenic line recovery, 501-502 reporter transgene design, 498-499 Calcineurin calcium/calmodulin activation, 71 oxidative stress reporter assay advantages and limitations, 80-81 buffers and media, 74-75 controls, 77-78

hydrogen peroxide-induced stress, 71, 78-80

luciferase reporter assay, 76 plasmids, 74, 79 principles, 73-74, 76-77 rationale, 70 T cell culture, 75 transfection, 75-76 structure, 71

Carbon monoxide, cellular defense functions,

374, 376 Catalase, assay in brain, 394 Caveolae caveolin-1 functions, 131, 133-134 cholesterol modulation caveolin-1 maintenance of levels, 133-134

cholesterol oxidase treatment studies, 135-136

cyclodextrin binding studies, 136-137 depletion effects on structure and function,

132-133 filipin binding studies, 134-135 quantitative analysis direct mass determination, 139 radioisotope labeling, 138-139 signal transduction, 133 definition, 131-132

oxidized low-density lipoprotein treatment studies, 137-138 structure, 131-132 CD, see Circular dichroism Chemokines classification, 460

glutathione peroxidase transgenic mouse studies construction, 463-464 kidney ischemia-reperfusion injury and chemokine analysis immunostaining, 468-469 mouse model, 464 Northern blot analysis, 466 nuclear factor-*: B electrophoretic mobility shift assay, 470-471 nuclear run-on transcriptional assay, 474

polysome profile analysis, 474-475

prospects for study, 476

reverse transcription-polymerase chain reaction, 465 RNase protection assay, 466, 468 Western blot of chemokines, 466,

469-470 Western blot of I-*:B, 471, 473-474 protective mechanisms, 475-476 rationale for generation, 462-463 oxidative stress and activation, 460-462,

470 receptors, 460

transcription factors in expression, 461-462 Cholesterol, see Caveolae Circular dichroism, redox probe-labeled oligodeoxynucleotides, 563-564 CNG channel, see Cyclic nucleotide-gated channel CO, see Carbon monoxide Copper-containing amine oxidase catalytic intermediates, 313-314 single-crystal ultraviolet-visible microspectrophotometry catalytic intermediate observations, 315 crystal preparation, 314-315 CPM, see 7-Diethylamino-3-

(4'-maleimidylphenyl)-4-methylcoumarin Cryoenzymology, see Nitric oxide synthase aB-Crystallin, transgenic mouse studies of myocardial ischemia-reperfusion injury rationale for transgenesis, 354-355 Southern blot analysis, 357, 359 transgenic mouse generation, 355, 357 Cyclic nucleotide-gated channel olfactory neuron channel drug preparation, 212-213 green fluorescent protein fusion protein expression and Northern blot, 216-217 neuron isolation from rat, 212 patch-clamp recordings, 212 S-nitrosylation controls, 214

nitric oxide donor perfusion, 213-214 site-directed mutagenesis of cysteine residues in «3 subunit, 211-212, 216

sites of modification and channel activation, 217-219 sulfhydryl reagent modification studies, 214,216 subunits, 211 sensory systems, 210 Cyclodextrin, caveolae binding studies,

136-137 Cysteine organic oxyacids and stability, 44-45, 48, 102

redox state, see Disulfide bond sulfenic acid, see NADH peroxidase Cysteine peroxidase, cysteine-sulfenic acid, 49-50

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