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Deoxyguanosine-rert-4-hydroxy-2-nonenal adduct, see ieri-4-Hydroxy-2-nonenal

2',7'-Dichlorofluorescein diacetate, superoxide assay for NADPH oxidase detection, 227-229

7-Diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin, ryanodine receptor modification in junctional sarcoplasmic reticulum fluorescence measurement, 243-244 fluorescent adduct, 241 kinetics of sulfhydryl modification, 241-242,

244, 246 membrane isolation, 242-243 redox sensor hyperreactive sulfhydryls, 252-253

Differential scanning calorimetry, redox probe-labeled oligodeoxynucleotides, 564

Dihydroethidium, superoxide assay for NADPH oxidase detection, 227-230

Disulfide bond computational prediction accessible surface area calculation, 13-14 amino acid residue abundance in proteins by type, 15 apolar residue interactions with free cysteines, 15-16 approaches, 11-12 conservation scores, 13-14, 20 cysteine and disulfide conservation and occurrence in secondary structures, 18-19 databases, 12-13

Internet access for prediction program, 21 misprediction types, 20-21 polar residue abundance around disulfides, 16

proteins containing both cysteines and half-cystines, 16-17, 19-20 one-electron redox intermediates in disulfide/dithiol couple, 35-36 radiolysis final products and chain length, 43 free radicals decay, 42 formation, 41-42 gamma radiolysis principles, 39-40

ionizing radiation interaction with aqueous solutions, 37-38 kinetic scheme, 36-37 pA"a determination for disulfide radical, 43

pulse radiolysis principles, 39 radiation conditions, 41 reagents, 40 sample preparation, 40 thioredoxin studies, 43-44 sequence information in prediction, 10-11 subcellular protein location correspondence with disulfide-bonding state, 17-18 DNA base mismatch, see Single-nucleotide polymorphism DNA hybridization arrays, see also Gene chip gene chips, 488 macroarrays, 487-488 microarrays, 488 microelectronic arrays, 488 Drug receptors, affinity bead isolation anti-nuclear factor-*: B drug receptor purification, 85-86 latex beads advantages, 83

agarose resin comparison, 86-87 composition, 81, 83 drug amino derivative coupling, 85 preparation, 84 spacer introduction, 84-85 overview, 83-84 troubleshooting drug coupling to beads, 87 protein recovery purity, 88 yield, 87-88 DSC, see Differential scanning calorimetry

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