Man

ECE, see Endothelin-converting enzyme Electron microscopy, immunogold staining of manganese superoxide dismutase in transgenic mice, 403-404 Electron spin resonance, superoxide assay for

NADPH oxidase detection, 231-232 Electrophoretic mobility shift assay nuclear factor-fcB, 470-471 topoisomerase lia, 3'-untranslated region binding to protein, 454-456

ELISA, see Enzyme-linked immunosorbent assay

ELISPOT, see Enzyme-linked immunospot assay

EMSA, see Electrophoretic mobility shift assay Endothelin-converting enzyme enzyme-linked immunosorbent assay of activity, 264-265 function, 263 isolation endothelial cell culture, 264 membrane purification, 264 reactive oxygen species effects dimerization induction, 266 radical donor incubation, 265-266 zinc content, 266-268 Enzyme-linked immunosorbent assay endothelin-converting enzyme activity, 264-265

macrophage scavenger receptor, 345 Enzyme-linked immunospot assay history of development, 24 thioredoxin and thioredoxin reductase induction coating of plates, 27 data presentation, 31-32 development of plates, 30 inducers of secretion, 31, 34-35 materials, 25-27 mononuclear cell and monocytes isolation from human blood, 28-29 plating, 29 stimulation, 29 principles, 24-25

spot evaluation and enumeration, 30 statistical analysis, 30-31 validation flow cytometry, 33-34 immunoprecipitation, 33 Western blot, 33 ER, see Estrogen receptor EST, see Expressed sequence tag Estrogen receptor allosteric regulation, 57 breast cancer overexpression, 58 electrospray ionization mass spectrometry of DNA-binding domain oxidation alkylation of free thiols, 68-69 calculation of cysteine oxidation degree, 65-66

high-performance liquid chromatography coupling, 59, 61, 64 instrumentation, 60-61 interpretation, 61-62 methionine oxidation analysis, 66-67 oxidation conditions, 63-64 rationale, 59

recombinant protein preparation, 62-63 tryptic digest preparation, 64 zinc binding stoichiometry determination, 67-68

metal substitution studies, 57-60 oxidative stress response, 58-59 zinc finger cooperativity in structure stabilization, 68 Expressed sequence tag cross-species comparisons, 567 database size, 567 error checking, 572-573 identification of human genes advantages, 567-568 Basic Local Alignment Search Tool, 569, 572-574

MRS4 homolog searching, 568-569, 574 Multiple Alignment program, 571-572 nr database searching, 573-574 proteome databases, 568-569 tag origin determination, 570-571 translation into protein sequence, 572-573 verification of in silico cloning, 574-575

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