Identification of Human Gene by Expressed Sequence Tag Database Searching

The EST database can also be exploited when positional cloning does not disclose one or more obvious candidate genes mapping in a genetic interval identified by linkage analysis. However, this approach is not so straightforward as for identification of a human gene by homology sequence with an as yet unknown sequence, as described above. Indeed, the researcher must continuously ply between computing and experimental work (YAC or PAC contig constitution, segregation analysis of polymorphic markers in families, search for transcribed sequences, etc.), which cannot be linearly described.

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