Kopen= 0.0032 sec-i

Kclosed= 0.0036 sec:L

100 200 300 400 500 Time, sec

Fig. 3. Kinetic measurement of forming CPM-thioether adducts with JSR and LSR. Data traces show that JSR rapidly forms an adduct with CPM in the presence of physiologic channel inhibitor Mg2+ (top) whereas LSR exhibits only slow adduct formation regardless of which modulator is present (bottom). Inset, top: SDS-PAGE separation of JSR labeled for 1 min with 10 nM CPM in the presence of either (A) 2 m M Mg2+ or (B) 100 jxM Ca2+.

cis- trans cis- trans

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