Fig. 1. Oxidation of BH4-free neuronal deoxyferrous NOS by O2 at —30° in the presence of 1 mM NHA. Shown are the spectra of dithionite-reduced BFLt-free NOS (dotted line), as well as the spectra recorded immediately (within 2 min, continuous line) and 43 min (dashed line) after O2 addition. Inset: The visible part of the spectra at greater magnification and vertically offset for clarity. Experimental conditions: 1.8 fiM NOS, 1 mM NHA, 50 mM kpi (pH 7.2), 1 mM CHAPS, 0.5 mM EDTA, 1 mM 2-mercaptoethanol, 0.23 mM sodium dithionite, and 50% (v/v) ethylene glycol. See Experimental Procedures for further details.

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