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photooxidation studies of charge transfer,

565-566 purification high-performance liquid chromatography, 559, 561

polyacrylamide gel electrophoresis, 561-562 rationale for synthesis, 548-549 synthesis anthraquinone phosphoramidite synthesis, 557-559

base labeling with ruthenium, 552-555 2'-deoxyribose labeling with phenothiazine, 555-557

direct incorporation of probes, 559 phosphoramidite synthetic approach,

550-551 strategies, 550 Ref-1

AP-1 sulfhydryl reduction, 3 hypoxia-inducible factor-like factor interactions, mammalian two-hybrid system cysteine reduction by Ref-1, 7-8 false-positives, 8-10 interpretation, 7 plasmids, 4, 6 principles, 4 transfection, 6-7 hypoxic protein response enhancement, 3-4

Reverse transcriptase-polymerase chain reaction chemokines, 465 heme oxygenase-1, 385-386 8-oxoguanine repair assay in human cells, 544-546

RT-PCR, see Reverse-transcription polymerase chain reaction Rubredoxin, neelaredoxin reduction, 151, 154 Ryanodine receptor 7-diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin studies of junctional sarcoplasmic reticulum fluorescence measurement, 243-244 fluorescent adduct, 241 kinetics of sulfhydryl modification,

241-242, 244, 246 membrane isolation, 242-243 redox sensor hyperreactive sulfhydryls, 252-253 isoforms, 240 redox sensing studies glutathione gradient gradient instillation across membrane,

248-250 redox potential determination, 248 stock solution preparation, 247-248 high open probability gating mode measurement, 250-252 reconstitution of RyRl in bilayer lipid membranes, 246-247 sulfhydryl-modifying reagent sensitivity, 240-241 RyR, see Ryanodine receptor

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