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Fig. 3. The strategy used for introduction of loxP sites by gene targeting of the Tfam locus. The targeting vector is composed of homologous sequences corresponding to the three 3' exons of Tfam, a positive selection marker (PGK-neo) and a negative selection marker (HSV-TK). Homologous recombination events will result in creation of the TfamloxP locus, where the last two exons of Tfam are flanked by loxP sites. The knockout allele Tfam~ is created by ere expression, which deletes the two exons flanked by loxP sites.

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