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FIG. 5. EKG analysis of coronary artery-induced ischemia and reperfusion. Schematic illustration of a normal EKG trace (A) and the diagnostic EKG changes characteristic of ischemia (B and C) and reperfusion (D). The two theoretical possibilities in (B) and (C) may occur depending on placement of the EKG leads and the channel analyzed. (E-G) EKG recordings performed on an anesthetized rat before ischemia (E), 10 min after the initiation of ischemia (F), and 2 min after the start of reperfusion (G). The recordings in (E) and (F) are from lead II in the same animal.

9-0 black monofilament nylon with CS-175-8 needle (Ethicon) Surgical instruments: Fine surgical scissors, fine forceps (curved or strait), mosquito hemostatic forceps (straight or curved), disposable scalpels (Feather Safety Razor, Osaka, Japan, Medical Division), small animal retractors, and modified surgical clamps (fine curved surgical forceps with the tips bent to 90° and covered with PE20 tubing) Hamilton microinjection syringe with a 33-gauge needle lOOCh

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