O

Oligonucleotides antisense oligodeoxyribonucleotide, see NADPH oxidase; Protein kinase C

high-density oligonucleotide array, see Gene chip redox probe labeling, see Redox probelabeled oligodeoxynucleotides Optically transparent thin-layer electrode advantages in heme protein studies, 192 cell construction, 191-192 detection systems, 194 electrode materials, 190-191 hemoglobin allosteric effector studies, 208 Nernst plots and data analysis, 204-205,

207-208 non-Nernstian response, 205-206 oxygenation relationship with redox properties, 206-207 mediator use and criteria, 193-194 myoglobin

Nernst equation, 194 Nernst plots and data analysis, 196-199 non-Nernstian response, 199-200 noninteracting electroactve centers model development, 200-204 myoglobin mixtures, 204 sample preparation, 195-196 spectra acquisition, 196 OTTLE, see Optically transparent thin-layer electrode Oxidative stress biotinylated glutathione ethyl ester labeling of proteins, see Biotinylated glutathione ethyl ester Caenorhabditis elegans gene response, see Caenorhabditis elegans oxidant stress-responsive genes damage of cell components, 101, 460 definition, 70

DNA lesions, see (<;/-i-4-Hydroxy-2-nonenal; 8-Oxoguanine; Redox probe-labeled oligodeoxynucleotides; Single-nucleotide polymorphism heme oxygenase modulation, see Heme oxygenase protein responses, see Calcineurin;

Chemokines; Endothelin-converting enzyme; Estrogen receptor; Integrins; Metallothioneins; 20S Proteasome; Thioredoxin; Thioredoxin reductase; Topoisomerase Ila; Vanillyl-alcohol oxidase

8-Oxoguanine BRCA lesions, 547 mutagenicity, 536

plasmid mutagenesis assay in replicating human cells electroporation of bacteria, 541-542 overview, 537

plasmid construction, 537, 539-541 rationale, 547

restriction enzyme analysis, 542 transfection, 541 plasmid transcription detection in human cells reverse transcription-polymerase chain reaction, 544-546 RNA isolation, 544 transcription-coupled repair defects in disease, 545-546 repair assay in human cells plasmid construction, 543 transcription-coupled repair frequency, 542, 544, 547

transfection, recovery, and amplification, 543-544 repair pathways, 536-537, 547

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