Oh

HNE-dG 5'-monophosphates

Scheme 2. Formation of HNE~dG adducts from reaction of HNE with DNA and reactions involved in their detection and identification. Two pairs of diastereomers of HNE-dG 3'-monophosphates are formed as indicated by HPLC (Fig. 1). However, only one of the two pairs is shown here. [Reproduced with permission from F.-L. Chung, R. G. Nath, J. E. Ocando, A. Nishikawa, and L. Zhang, Cancer Res. 60, 1507 (2000).]

b Standard 1+2

34 i

Fig. 1. Detection of HNE-dG adducts as 3',5'-bisphosphates in rat and human tissues. These adducts are formed as two pairs of diastereomers 1,2 and 3,4 and isomers 1 and 2 at the 3',5-bisphosphate level are not separated under the HPLC conditions used. The identities of the radioactive peaks obtained by 32P-postlabeling are indicated by their HPLC comigration with the synthetic UV markers, (a) A blank sample of poly(dA-dC) : poly(dG-dT) is used to ensure that the assay is free of contamination; (b) external standards of HNE-dG adducts are used for quality assurance and quantitation; (c) rat tissues; (d) human tissues. [Reproduced with permission from F.-L. Chung, R. G. Nath, J. E. Ocando, A. Nishikawa, and L. Zhang, Cancer Res. 60, 1507 (2000).]

Rat liver il

Human liver jl

10 Ways To Fight Off Cancer

10 Ways To Fight Off Cancer

Learning About 10 Ways Fight Off Cancer Can Have Amazing Benefits For Your Life The Best Tips On How To Keep This Killer At Bay Discovering that you or a loved one has cancer can be utterly terrifying. All the same, once you comprehend the causes of cancer and learn how to reverse those causes, you or your loved one may have more than a fighting chance of beating out cancer.

Get My Free Ebook


Post a comment