P

PCR, see Polymerase chain reaction Periplasmic redox protein localization, see Lysozyme-osmotic shock Phospholipase A2

antisense knockdown effects on superoxide production, 432-433 NADPH oxidase regulation, 432-433 PKC, see Protein kinase C PLA2, see Phospholipase A2 Plasmacytoma, see B cell Polymerase chain reaction, see also Reverse-transcription polymerase chain reaction heme oxygenase-1 transgenic mouse phenotyping, 387-388 huntingtin transgene detection, 367-368 macrophage scavenger receptor DNA, 344 manganese superoxide dismutase transgenic mouse genotyping, 401-402 metallothionein I promoter, 481 20S Proteasome isolation activation conditions, 256-257

ammonium sulfate precipitation and conventional chromatography, 255 overview, 254-255

ultracentrifugation and chromatography, 255-256 oxidation-mediated effects metal-catalyzed oxidation effects on activity cell studies, 260-261 purified protein, 258 prospects for study, 262 proteolytic assays, 257-258 structural changes carbonyl group detection, 261-262 two-dimensional gel electrophoresis, 261

ultraviolet-A oxidation effects on activity, 259-260

oxidized protein degradation, 253-254 subunits, 254 Protein kinase C

phosphorylative activation, 90 S-glutathiolation of PKCa fibroblast assay activity assay, 98 anion-exchange chromatography of extract, 97-98 diamide incubation, 97 dithiothreitol reversibility, 98-99 immunoprecipitation and Western blot analysis, 100 NIH 3T3 cell culture, 97-98 oxidant-induced thiolation assay,

98-100 principles, 97 inactivation of enzyme, 90 purified protein studies activity assay, 93 diamide induction, 91-93 dithiothreitol reversibility, 93-94 gel electrophoresis and autoradiography analysis, 94-96 stoichiometry of modification, 96-97 thiol reduction, 90-91 Protein kinase C

antisense knockdown effects on superoxide production, 432-433 isozymes, 432

NADPH oxidase expression regulation, 432

Pulse radiolysis, see Radiolysis pUR288 transgenic shuttle vector assay, see B cell

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