Sir2

assay of histone deacetylase activity acetylated histone purification from chicken, 298-299 incubation conditions, 299 catalytic mechanism, 288 cloning of homologs, 293 conserved sequence regions, 283, 288 crystal structure from Archaeoglobus fulgidus, 290-291

expression and purification from recombinant Escherichia coli Archaeoglobus fulgidus protein,

294-295 buffers and solutions, 297-298 glutathione S-transferase fusion proteins from humans and yeast, 296-297 Thermotoga maritima protein, 295-296 gene silencing assays colony color assays, 293 overview, 291 spot assays, 291-292 nicotinamide adenine dinucleotide gene silencing regulation, 291 measurement in intact yeast, 299-300 sequence alignment across species,

284-287 yeast protein deacetylase activity and targets, 288-289 homologs in yeast, 283 transcriptional silencing role, 283 S-nitrosylation cyclic nucleotide-gated channel in olfactory neurons controls, 214

nitric oxide donor perfusion, 213-214 site-directed mutagenesis of cysteine residues in a3 subunit, 211-212, 216 sites of modification and channel activation, 217-219 sulfhydryl reagent modification studies, 214,216 enzyme targets, 209 metallothioneins cell studies and free thiol determination,

275-277, 279 direct analysis, 281

sulfhydryl regeneration with dihydrolipoic acid, 272-273 SNP, see Single-nucleotide polymorphism

SOD, see Superoxide dismutase Southern blot aB-crystallin gene in transgenic mice, 357, 359

heat shock protein 70 gene in transgenic mice, 357, 359

homologous recombination screening in transgenic mice, 414-415, 417 manganese superoxide dismutase transgenic mouse genotyping, 400, 402 S-thiolation endogenous thiolating species, 89 glutathiolation, see Protein kinase C oxidative stimuli, 89 Sulfenic acid, see NADH peroxidase Superoxide assay in NADPH knockdown studies, 428 cell damage and pathology, 263 endothelin-converting enzyme, effects on zinc content, 266-267 NADPH oxidase assay aconitase, 232-233 chemiluminescence, 226-227 cytochrome c reduction, 225-226 2',7'-dichlorofluorescein diacetate,

227-229 dihydroethidium, 227-230 electron spin resonance, 231-232 nitroblue tetrazolium, 230-231 phagocytic oxidase considerations, 224-225 Superoxide dismutase expression regulation, 407-408 isoforms, 349

manganese superoxide dismutase transgenic mice activity gel, 403 antioxidant status assessment enzymes, 404-405 low-molecular-weight antioxidants, 405-406 essentiality of enzyme, 398 generation, 399-400 genotyping polymerase chain reaction, 401-402 Southern blot, 400, 402 immunogold staining, 403-404 Northern analysis, 402 oxidative stress protection studies, 406-409

rationale for generation, 398-399 tissue distribution of expression, 408 Western blot, 402 metallothionein copper transfer to apo-copper/zinc superoxide dismutase, 273, 275

myocardial ischemia-reperfusion injury, copper/zinc-superoxide dismutase knockout in mice heterozygote phenotype, 351 rationale, 349-350 targeted gene disruption, 350-351 neonatal stroke hypoxia-ischemia model, copper/zinc-superoxide dismutase transgenic mouse overexpression adult versus neonatal findings,

390-391 enzyme assays in brain catalase, 394

glutathione peroxidase, 394 hydrogen peroxide, 394-395 histology brain sample preparation,

391-392 cresyl violet staining, 392 Perl's iron stain, 392-393 hydrogen peroxide accumulation in neurotoxicity, 396-397 hypoxia-ischemia model modifications, 390

neuron culture studies cell culture, 395-396 overview, 395 stroke response of expression, 397 Superoxide reductase, see Neelaredoxin Syphilis, see Neelaredoxin; Treponema pallidum

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