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Figure 4 Plasma retention of NO (open circles) and heme (closed circles) of SNO-PEG-Hb in the circulation of rats after a bolus injection at 125 mg/kg.

was used10. After blood was removed (up to 30%) from anesthetized male Wistarrats under 21% 02 ventilation, saline, PEG-Hb or SNO-PEG-Hb (5% Hb solution) was isovolumetrically infused. The p02 in the cervical vein and arterial blood pressure were monitored throughout the experiments. The intravenous infusion of SNO-PEG-Hb and PEG-Hb restored oxy-Hb, total-Hb, and cytochrome oxidase reduction levels of cerebral tissues (Fig. 5). This suggested that SNO-PEG-Hb could supply enough oxygen to the brain, like PEG-Hb. During the Hb infusion, PEG-Hb could quickly restore blood pressure, while this recovery was slower after the infusion of SNO-PEG-Hb. This suggested that SNO-PEG-Hb causes vasodilation as an NO donor.

The redox status of cytochrome oxidase in the rat brain after hemorrhagic shock and Hb infusion. (A) "typical tracings; (B) Comparison at the end of Hb infusion. The duration of hemorrhage and transfusion was indicated by bars. The relative change against the value obtained during the resting period is indicated in some figures.

Figure 5

The redox status of cytochrome oxidase in the rat brain after hemorrhagic shock and Hb infusion. (A) "typical tracings; (B) Comparison at the end of Hb infusion. The duration of hemorrhage and transfusion was indicated by bars. The relative change against the value obtained during the resting period is indicated in some figures.

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