Pegmethioninase

Elevated minimum methionine dependence of most and possibly all types of tumor cells relative to normal cells has been demonstrated16-27. The L-methionine a-deamino-y-mercaptomethane lyase (methioninase, METase) gene from Pseudomonas putida has been previously cloned in Escherichia coli27-29. Recombinant METase (rMETase) is a homotetrameric pyridoxal 51-phosphate enzyme of 172 kDa molecular mass.

The biochemical reaction catalyzed by L-methioninase is shown below:

The biochemical reaction catalyzed by L-methioninase is shown below:

A scale-up rMETase production protocol has been established with high yield (60%), high purity (98%), high stability, and low endotoxin for preclinical and clinical studies targeting the methionine dependence of human cancer27.

Studies of the antitumor efficacy of rMETase in vitro and in vivo on human tumors xenografted in nude mice demonstrated that all types of

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