Definition of Protein Solubility

Protein solubility is a thermodynamic characteristic of the protein/solvent system defined as the concentration of soluble protein in equilibrium with the solid phase at a given pH, temperature, and solvent composition (Flynn, 1984; Arakawa and Timasheff, 1985; Middaugh and Volkin, 1992). For practical purposes, solubility of proteins can be defined as the maximum amount of protein that remains in a visibly clear solution (i.e. does not show protein precipitates, crystals, gels, or hazy soluble aggregates), or does not sediment at 30,000 g cen-trifugation for 30 min (Schein, 1990; Ducruix and Reis-Kautt, 1990).

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