Chlorpromazine is a cationic amphiphilic drug that prevents recycling of receptors for transferrin, LDL, and EGF (Wang et al. 1993). Chlorpromazine and related drugs decrease the amount of clathrin-coated pits on the cell surface. They also cause coated pits to assemble on endosomes and relocation of the AP-2 complex from the plasma membrane to the cytoplasm and to cytoplasmic vesicles of unknown origin or identity. As with other endocytosis inhibitors, the effects appear to be reversible. Given that cationic amphiphilic drugs affect the activity of many different enzymes like phosphatadic acid phosphorylase, protein kinase C, or Ca+/ calmodulin-dependent enzymes (Wang et al. 1993), inhibition of clathrin-mediated endocytosis will almost certainly be accompanied by perturbation of other metabolic pathways.

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