EVH2 Domain Structure and Function

The EVH2 domain contains binding sites for G-actin (Walders-Harbeck et al. 2002; Chereau and Dominguez 2006) and F-actin (Bachmann et al. 1999; Huttelmaier et al. 1999; Harbeck et al. 2000). The most C-terminal part of the EVH2 domain

Table 1 EVH1 class-I-domain-binding sites found in different ligand proteins

Listeria monocytogenes ActA

(Niebuhr et al. 1997)

Human Fat-1

(Moeller et al. 2004; Niebuhr et al 1997; Tanoue and Takeichi 2004) Human Fyb/SLAP

(Krause et al. 2000) Human Lamellipodin (Krause et al. 2004)

Migfilin

(Zhang et al. 2006) Palladin

(Boukhelifa et al. 2004) Human RIAM

(Lafuente et al. 2004)

Drosophila Robo

(Bashaw et al. 2000) Human Vinculin

(Brindle et al. 1996; Reinhard et al. 1996) Human Xin

(van der Ven et al. 2006) Human Zyxin (Drees et al. 2000)

DFPPPPTDEEL EFPPPPTDEEL EFPPPPTEDEL DFPPIPTEEEL DFPPPPED

IFPPPPDDDIYDG

QFPPPPTPPAME

DFPPPPPESSL

VFPPPPPSPVP

EFPSPPSDSDF

DFPPPPPETDL

DLPLPPIE

MALPPPPPEL

DLLPPPPPPPPVLLPSEEE

PFPPPPPAFPEL

DVFPLPPPPPPLP

DDLPPPPAD

DLPLPPPPPEPL

SLPPPPPVRR

DDFLPPPPPPPPLDD

ELPPPPPD

DFVPPPPPSYA

ELPPPPPPPAP

EFLPPPPEHPPP

PDFPPPPPDLEQLR

EDLPLPPPPALEDL

EDFPLPPPPLAGDG GAFPPPPPPMIEEP ESFPPAPLEEEIFP EIFPSPPPPPEEEG

contains a coiled-coil motif that mediates tetramerisation of Ena/VASP proteins (Bachmann et al. 1999; Barzik et al. 2005; Kuhnel et al. 2004; Zimmermann et al. 2002). The main molecular function of Ena/VASP proteins is to allow elongation of actin filaments by protecting them from being capped by capping protein. The G- and F-actin-binding sites and the coiled-coil motif are required for this function

(Barzik et al. 2005; Bear et al. 2002). This anti-capping activity is potentiated by the recruitment of profilin to its binding site in the central proline-rich region (Barzik et al. 2005).

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