Chromatin immunoprecipitation (ChIP) is a method used to determine the location of DNA binding sites on the genome for a particular protein of interest. This technique gives a picture of the protein-DNA interactions that occur inside the nucleus of living cells or tissues. The principle underpinning this assay is that DNA-binding proteins (including transcription factors) in living cells can be cross-linked (often using formaldehyde) to the DNA to which they are binding. By using an antibody that is specific to a putative DNA binding protein, one can immunoprecipitate the protein-DNA complex out of cellular lysates.
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