Grow bacteria to obtain required gene

Copyright <ยง American Psychiatric Publishing, Inc., or American Psychiatric Association, unless otherwise indicated in figure legend, All rights reserved.

See text for details.

The cloning of disease-related genes is an important step toward insight into the pathogenesis of diseases and development of new drugs against these diseases. If a protein is suspected of involvement in pathogenesis, a nucleic acid sequence can be deduced from the partial amino acid sequence. cDNA libraries are screened with the partial sequence in order to fish out a complete clone of interest. The isolated cDNA molecules are used to infect bacteria, which amplify the cloned cDNA. Bacteria expressing these cDNA molecules will often make the protein of interest, which can be detected with antibody. A variation on the above technique uses antibodies against the purified proteins to screen a DNA library transfected into bacteriophages. The bacteriophages containing the "right" cDNA can be retrieved, and the sequence of cDNA can be analyzed.

Was this article helpful?

0 0
Nicotine Support Superstar

Nicotine Support Superstar

Stop Nicotine Addiction Is Not Easy, But You Can Do It. Discover How To Have The Best Chance Of Quitting Nicotine And Dramatically Improve Your Quality Of Your Life Today. Finally You Can Fully Equip Yourself With These Must know Blue Print To Stop Nicotine Addiction And Live An Exciting Life You Deserve!

Get My Free Ebook

Post a comment