Grow bacteria to obtain required gene

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The cloning of disease-related genes is an important step toward insight into the pathogenesis of diseases and development of new drugs against these diseases. If a protein is suspected of involvement in pathogenesis, a nucleic acid sequence can be deduced from the partial amino acid sequence. cDNA libraries are screened with the partial sequence in order to fish out a complete clone of interest. The isolated cDNA molecules are used to infect bacteria, which amplify the cloned cDNA. Bacteria expressing these cDNA molecules will often make the protein of interest, which can be detected with antibody. A variation on the above technique uses antibodies against the purified proteins to screen a DNA library transfected into bacteriophages. The bacteriophages containing the "right" cDNA can be retrieved, and the sequence of cDNA can be analyzed.

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