Chemical Details

SAMPLE ORIGIN SAMPLE PREP./SIZE PYROL. CONDITIONS TOTAL ION COUNTS

ADRENOCORTICOTROPHIC HORMONE, fragment ACTH 1-24

H-Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val-Gly-Lys-Lys-Arg-Arg-Pro-Val-Lys-Val-Tyr-Pro-OH

gift from Dr. H.M. Greven, suspension in PBS; 10 yg standard 2 X 105

Organon, Oss, The Netherlands

REMARKS - The almost complete absence of a peak at m/z 34 (H2S+-) is due to the lack of Cys residues. The relatively small amount of H2S is formed from Met residues (ref. 58) which mainly yields CH3SH+" (m/z 48). The prominent peak at m/z 69 points to the high Lys and Pro contents. The peak at m/z 60 (in combination with m/z 43) is thought to represent acetic acid derived from the acetate counter ions on the NH2-functions of the Lys and Arg residues. The relatively intense peak at m/z 117 in combination with the peak at m/z 131 is mainly due to (methyl)indole derived from the Trp residue.

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