Sample

CHEMICAL DETAILS SAMPLE ORIGIN SAMPLE PREP./SIZE PYROL. CONDITIONS TOTAL ION COUNTS

HEMOGLOBIN (from bovine erythrocytes) for amino acid composition see Table 13 J.T. Baker Chemicals, Phillipsburg, NJ, USA suspension in methanol; 10 yg standard 1 X 105

remarks - This protein shows a rather balanced pattern. Specially noteworthy is the obvious absence of peaks which can be directly ascribed to the heme moiety. However, it should be realized that the porphyrin unit represents only about 1% by weight of the organic material. Moreover, the heme moiety tends not to give characteristic fragments, e.g. pyrrole and pyrroline derivatives, such as formed from bilirubin (Spectrum G.10).

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