Sample

CHEMICAL DETAILS SAMPLE ORIGIN SAMPLE PREP./SIZE PYROL. CONDITIONS TOTAL ION COUNTS

RIBONUCLEASE-A (from bovine pancreas) for amino acid composition see Table 13 Boehringer, Mannheim, GFR suspension-in methanol; 10 yg standard 1.1 X 105

remarks - The absence of Trp residues (producing indole and Me-indole) is indicated by the very low peaks at m/z 117 and 131, whereas the high abundance of Cys, and to a lesser extent Met residues,is evidenced by the high intensities at m/z 34 (HpS4"") and 48 (CH3SH+"). Although most RNase samples contain a few percent of hexose and N-acetylhexosamine residues, this carbohydrate content does not contribute significantly to the spectrum as judged by the absence of pronounced peaks at m/z 32 and 126.

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