CHEMICAL DETAILS SAMPLE ORIGIN SAMPLE PREP./SIZE PYROL. CONDITIONS TOTAL ION COUNTS
: GANGLIOSIDE GTlb (Trisialosyl-tetraglycosylceramide) : see insert in spectrum
: gift from Dr. R.W. Veh, Ruhr Universität, Bochum, GFR : suspension in PBS buffer; 10 yg : standard : 3 X 105
REMARKS - Sample prepared from bovine brain. Compared with the pattern of the asialo-analogue (Spectrum A.26) the spectrum of this ganglioside shows strongly increased signal intensities at m/z 59, 67, 123, 135 and 151. This appears to reflect the presence of the N-acetylneuraminic acid (sialic acid) residues (see Spectrum A.23). Furthermore, the lower intensities at m/z 97, 98, 110, 125 and 126 reflect the decreased percentage of hexose and N-acetylhexosamine residues. As in the case of Spectrum A.26 the lipid moiety is hardly expressed in the spectrum.
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