Sample

CHEMICAL DETAILS SAMPLE ORIGIN SAMPLE PREP./SIZE PYROL. CONDITIONS TOTAL ION COUNTS

NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE, reduced (NADPH)

see insert in spectrum; C21H26N7Na4°17P3; Boehringer, Mannheim, GFR suspension in methanol, 10 pg standard 3 X 105

REMARKS - Apart from the typical ribose fragment series (compare Spectra C.l, C.3) this spectrum shows marked contributions from the nicotinamide (m/z 79, 95, 104, 106, 122) and adenine (m/z 135) moieties. The presence of adenine is especially noteworthy since no adenine signal is observed in Py-mass spectra of DNA or AMP (see Spectra C.2-C.4). Apparently, in the latter cases no free base moieties are produced as discussed in Part I, Section 3.3.

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