Sample

CHEMICAL DETAILS SAMPLE ORIGIN SAMPLE PREP./SIZE PYROL. CONDITIONS TOTAL ION COUNTS

ADRENOCORTICOTROPHIC HORMONE, fragment ACTH 1-10

H-Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-OH

gift from Dr. H.M. Greven, Organon, Oss, The Netherlands suspension in PBS; 10 pg standard

2 X 105

REMARKS - Compare with Spectra B.l and B.3. This spectrum is now entirely dominated by massive Met and Trp fragment peaks at m/z 48 (methanethiol) and m/z 117 (indole), respectively. The acetic acid peaks (m/z 60, 43) are reduced with respect to Spectrum B.l due to the absence of Lys and the lower Arg content. The small peak at m/z 64 may point to slight oxidation of Met residues (ref. 96).

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