Sample

CHEMICAL DETAILS SAMPLE ORIGIN SAMPLE PREP./SIZE PYROL. CONDITIONS TOTAL ION COUNTS

COLLAGEN (from bovine Achilles tendon) for amino acid composition see Table 13 Koch-Light Ltd., Colnbrook, Bucks., UK suspension in methanol; 10 yg standard 6 X 104

REMARKS'- This spectral pattern is very unusual compared to those of most other proteins due to the extremely high content of Gly, Ala, Pro and Hyp residues, the complete absence of Trp and the very low contributions of aromatic and S-containing amino acid residues. The aliphatic character of this protein causes the dominance of small pyrolysis and EI fragments (with exception of the marked pyrrole and dihydropyrrole peaks at m/z 67, 69 and 83 from the Pro, Lys and Hyp moieties). Small amounts of carbohydrate are indicated by peaks at m/z 31, 32, 96, 98, 110 and 124.

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