SAMPLE PREP./SIZE PYROL. CONDITIONS TOTAL ION COUNTS
PEPTIDOGLYCAN (from Bacillus subtilis)
poly[(l-*4)DGlcNAcg(l->4)MurNAcB] with Ala-Glu-diaminopimelic acid-Ala cross-links, bound to the polysaccharide chains via the muramic acid carbonyl groups; see insert in spectrum A.25
gift from Dr. J.T.M. Wouters, Lab. of Microbiology, Univ. of Amsterdam, The Netherlands suspension in PBS buffer; 10 yg standard
REMARKS - Sample prepared following ref. 172. In comparison with the spectrum of the methanol suspension (Spectrum A.25) this spectrum shows a marked peak at m/z 55 possibly derived from the aliphatic amino acid and/or the Mur-lactic acid moieties. Also note the increased intensity at m/z 125 as well as the decreased intensities at m/z 95 and 109 as compared to Spectrum A.25. Peaks at m/z 36, 38 represent HC1+" (PBS buffer).
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