Sample Origin

SAMPLE PREP./SIZE PYROL. CONDITIONS TOTAL ION COUNTS

PEPTIDOGLYCAN (from Bacillus subtilis)

poly[(l-*4)DGlcNAcg(l->4)MurNAcB] with Ala-Glu-diaminopimelic acid-Ala cross-links, bound to the polysaccharide chains via the muramic acid carbonyl groups; see insert in spectrum A.25

gift from Dr. J.T.M. Wouters, Lab. of Microbiology, Univ. of Amsterdam, The Netherlands suspension in PBS buffer; 10 yg standard

1 X 105

REMARKS - Sample prepared following ref. 172. In comparison with the spectrum of the methanol suspension (Spectrum A.25) this spectrum shows a marked peak at m/z 55 possibly derived from the aliphatic amino acid and/or the Mur-lactic acid moieties. Also note the increased intensity at m/z 125 as well as the decreased intensities at m/z 95 and 109 as compared to Spectrum A.25. Peaks at m/z 36, 38 represent HC1+" (PBS buffer).

Oal/3(l--3)GalNAc/?!l—4)Gal/?(l-»4)Glc/3-ceramide

0 0

Post a comment